|
KMID : 0848120060310010011
|
|
International Journal of Oral Biology
2006 Volume.31 No. 1 p.11 ~ p.14
|
|
|
Identification and Detection of Streptococcus anginosus Using Species-Specific 16S rDNA Primers
|
|
Cho Ji-Sun
Yoo So-Young
Kim Hwa-Sook
Hwang Ho-Keel
Min Jeong-Beom
Kim Byung-Hoon
Baek Dong-Heon
Shin Hwan-Seon
Kook Joong-Ki
|
|
|
Abstract
|
|
|
|
This study was undertaken to develop PCR primers for the identification and detection of Streptococcus anginosus using species-specific forward and reverse primers. These primers targeted the variable regions of the 16S ribosomal RNA coding gene(rDNA). The primer specificity was tested against 12 S. anginosus strains and 6 different species(10 strains) of oral bacteria. The primer sensitivity was determined by testing serial dilutions of the purified genomic DNA of S. anginosus ATCC 33397T. The data showed that species-specific amplicons were obtained from all the S. anginosus strains tested, but not in the six other species. The PCR could detect as little as 0.4pg of the chromosomal DNA from S. anginosus. This suggests that the PCR primers are highly sensitive and applicable to the detection and identification of S. anginosus.
|
|
|
KEYWORD
|
|
|
Identification, PCR primer, Streptococcus anginosus, 16S rDNA
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|